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Counting Molecules in cell-free DNA and single cells RNA
澳门新葡8455最新网站:2016年07月05日 00:00 点击数:

报告人:Kasper Karlsson

报告地点:澳门新葡8455最新网站317室

报告澳门新葡8455最新网站:2016年07月08日星期五10:00-11:00

邀请人:

报告摘要:

Sequencing the human genome took 13 years to complete. The same sequencing effort can now be done in days. The increased sequening capability has allowed increased resolution, like the sequencing of single cells. However current sequencing technologies suffer from a higher error rate and often requires amplification of the input DNA or RNA, which introduces errors like nucleotide substitutions and an amplification bias where some molecules amplifies better than other. Here I will introduce my work in developing the Unique Molecular Identifier to both correct for errors and the amplification bias. I will also discuss my work with amplification-free sequencing of cell-free DNA in the setting of Non-Invasive Prenatal Diagnostics as well my efforts to understand RNA isoforms in single cells.

主讲人概况:

Kasper Karlsson received his PhD from the Karolinska Institutet, Stockholm, Sweden. He has done excellent work in single cell RNA sequencing. He published his work in Nature methods, Genomics and Genome Biology. He is currently a postdoc scholar with the departments of medicine and genetics, Stanford University, US. His current research interests include tumor mutation rates and initiation of metastasis.

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